A SYBR inexperienced I real-time polymerase chain response (PCR) assay for detection and quantification of Trichomonas gallinae
Trichomonas gallinae are parasitic flagellates of significance in wild and residential birds. The parasite is worldwide distributed, and Columbine birds are its foremost host. Current evaluation focuses completely on epidemiological and phylogenetic analysis. Nonetheless, there could also be nonetheless a lack of knowledge regarding parasite-host interaction or treatment progress.
Precise-time PCR is a helpful gizmo for diagnostic and quantification of gene copies in a determined sample. By amplification of a 113-bp space of the 18S small subunit ribosomal RNA gene, a SYBR green-based real-time PCR assay was developed.
An everyday curve was prepared for quantification analysis. Assay effectivity, linearity, and dissociation analysis have been effectively carried out. Specificity, sensibility, and reproducibility analysis have been examined. This assay could be a helpful gizmo not only for diagnostic features however moreover for future in vivo and in vitro T. gallinae analysis.
Buffer: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Description: A polyclonal antibody against GPR12. Recognizes GPR12 from Human. This antibody is Unconjugated. Tested in the following application: WB, IF, ELISA;WB:1/500-1/2000.IF:1/200-1/1000.ELISA:1/5000
Buffer: Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, pH 7.4 >95%, Protein G purified
Description: A polyclonal antibody against GPR12. Recognizes GPR12 from Human. This antibody is Unconjugated. Tested in the following application: ELISA, WB, IHC, IF; Recommended dilution: WB:1:500-1:5000, IHC:1:20-1:200, IF:1:50-1:200
Immunogen information: Synthesized peptide derived from the N-terminal region of human GPR12 at AA rangle: 10-90
Applications tips:
Description: A polyclonal antibody for detection of GPR12 from Human. This GPR12 antibody is for WB, IF, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from the N-terminal region of human GPR12 at AA rangle: 10-90
Immunogen information: Synthesized peptide derived from the N-terminal region of human GPR12 at AA rangle: 10-90
Applications tips:
Description: A polyclonal antibody for detection of GPR12 from Human. This GPR12 antibody is for WB, IF, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from the N-terminal region of human GPR12 at AA rangle: 10-90
Immunogen information: Synthesized peptide derived from the N-terminal region of human GPR12 at AA rangle: 10-90
Applications tips:
Description: A polyclonal antibody for detection of GPR12 from Human. This GPR12 antibody is for WB, IF, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from the N-terminal region of human GPR12 at AA rangle: 10-90
Buffer: Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, pH 7.4 >95%, Protein G purified
Description: A polyclonal antibody against GPR12. Recognizes GPR12 from Human. This antibody is HRP conjugated. Tested in the following application: ELISA
Buffer: Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, pH 7.4 >95%, Protein G purified
Description: A polyclonal antibody against GPR12. Recognizes GPR12 from Human. This antibody is FITC conjugated. Tested in the following application: ELISA
Buffer: Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, pH 7.4 >95%, Protein G purified
Description: A polyclonal antibody against GPR12. Recognizes GPR12 from Human. This antibody is Biotin conjugated. Tested in the following application: ELISA
Description: A polyclonal antibody raised in Rabbit that recognizes and binds to Human GPR12 (Extracellular Domain). This antibody is tested and proven to work in the following applications:
Description: A polyclonal antibody raised in Rabbit that recognizes and binds to Human GPR12 (N-Terminus). This antibody is tested and proven to work in the following applications:
Description: A polyclonal antibody raised in Rabbit that recognizes and binds to Human GPR12 - C-terminal region. This antibody is tested and proven to work in the following applications:
Enchancment of a typical RT-PCR assay for grapevine vitiviruses
The genus Vitivirus throughout the family Betaflexiviridae incorporates eleven viruses acknowledged to infect grapevine: grapevine vitiviruses A, B, D, E, F, G, H, I, J, L and M (GVA-GVM). Three of these viruses, GVA, GVB and GVD, have been associated to the etiology of rugose wood sickness in grapevine and set off agronomically important losses. The other vitiviruses have been additional simply recently discovered and their outcomes on grapevine are undetermined. To certify grape supplies for propagation as virus examined, an updated reverse transcription PCR (RT-PCR) assay to detect all acknowledged vitiviruses is fascinating.
To carry out this, a variety of grapevine vitivirus sequences have been aligned on the amino acid diploma to hunt for conserved motifs. Two extraordinarily conserved motifs have been found at a superb distance for RT-PCR detection throughout the RNA-dependent RNA polymerase space of the replicase protein. The amino acid motifs have been once more translated to create degenerate primers and used to effectively amplify all eleven grapevine vitiviruses. The RT-PCR primers have been used to verify a panel of vitivirus-infected vines for inclusivity along with vines contaminated with fastidiously related viruses throughout the Betaflexiviridae family (i.e. grapevi
ne pinot gris virus and grapevine rupestris stem pitting-associated virus) for exclusivity. Broader use of these primers to detect vitiviruses in numerous plant hosts was investigated. In summary, an end-point RT-PCR assay that detects the entire acknowledged grapevine vitiviruses and doubtless completely different members of the genus Vitivirus has been developed. The widespread assay represents another option to explicit individual assays to cut back the work associated to the prognosis of vitiviruses, along with for regulatory features.
Pathologic full response (pCR) prices and outcomes after neoadjuvantchemoradiotherapy with proton or photon radiation for adenocarcinomas of the esophagus and gastroesophageal junction
Background: Pathologic full response (pCR) after neoadjuvantchemoradiotherapy (nCRT) is expounded to improved survival in victims dealt with for esophageal most cancers. Whereas proton beam treatment (PBT) has been demonstrated to cut back toxicities with nCRT, no info evaluating pCR prices between modalities exist to date. We investigated pCR prices in victims with distal esophageal/GEJ adenocarcinomas current course of trimodality treatment with nCRT-PBT or photon-based nCRT with the hypothesis that pathologic responses with PBT is usually a minimal of as extreme as with photon treatment.
Methods: A single-institutional consider of victims with distal esophageal adenocarcinoma dealt with with trimodality treatment from 2015-2018 using PBT was achieved. PBT victims have been matched 1:2 to victims dealt with with photons. Chi sq. and two-sample t-tests have been utilized to verify traits, and the Kaplan Meier approach was used to estimate oncologic endpoints.
Outcomes: Eighteen consecutive PBT victims have been acknowledged and as compared with 36 photon victims. All victims obtained concurrent chemotherapy; 98% with carboplatin/paclitaxel. Most victims have been male (91%) and White (89%); median age was 62 years (fluctuate, 31-76 years). Median radiation dose in every cohorts was 50.4 Gy (fluctuate, 41.4-50.4 Gy); all packages have been delivered in 1.8Gy fractions. Age, gender and race have been successfully balanced.
Victims dealt with with PBT had a significantly better pre-treatment nodal stage (N) and AJCC 7th model stage grouping (P=0.02, P=0.03). No matter this, tumoral and nodal clearance and pCR prices have been equal between cohorts (P=0.66, P=0.11, P=0.63, respectively). Whole pCR and explicit individual fundamental and nodal clearance prices, complete survival (OS), locoregional administration (LRC), and distant metastatic administration did not significantly differ between modalities (all P>0.05). Principal perioperative events have been balanced; however, there have been 5 (14%) perioperative deaths throughout the photon cohort as compared with 0 (0%) throughout the proton cohort (P=0.06).
Conclusions: Utilizing PBT in trimodality treatment for distal esophageal adenocarcinoma yields pCR prices just like photon radiation and historic controls. Pathologic responses and oncologic outcomes on this analysis did not differ significantly between modalities no matter PBT victims having better AJCC ranges and nodal sickness burdens.
Description: A polyclonal antibody raised in Goat that recognizes and binds to Human Goat Anti-GPR81 / FKSG80 . This antibody is tested and proven to work in the following applications:
Description: A polyclonal antibody raised in Rabbit that recognizes and binds to Human GPR81 (C-term). This antibody is tested and proven to work in the following applications:
Description: A polyclonal antibody raised in Rabbit that recognizes and binds to Human FKSG80 / GPR81 (C-Terminus). This antibody is tested and proven to work in the following applications:
Description: A polyclonal antibody raised in Rabbit that recognizes and binds to Human FKSG80 / GPR81 (Extracellular Domain). This antibody is tested and proven to work in the following applications:
Description: A polyclonal antibody raised in Rabbit that recognizes and binds to Human FKSG80 / GPR81 (N-Terminus). This antibody is tested and proven to work in the following applications:
Description: This gene is a member of the septin family of GTPases. Members of this family are required for cytokinesis. One version of pediatric acute myeloid leukemia is the result of a reciprocal translocation between chromosomes 11 and X, with the breakpoint associated with the genes encoding the mixed-lineage leukemia and septin 2 proteins. This gene encodes four transcript variants encoding three distinct isoforms. An additional transcript variant has been identified, but its biological validity has not been determined.
Description: This gene is a member of the septin family involved in cytokinesis and cell cycle control. This gene is a candidate for the ovarian tumor suppressor gene. Mutations in this gene cause hereditary neuralgic amyotrophy, also known as neuritis with brachial predilection. A chromosomal translocation involving this gene on chromosome 17 and the MLL gene on chromosome 11 results in acute myelomonocytic leukemia. Multiple alternatively spliced transcript variants encoding different isoforms have been described.
Description: This gene is a member of the septin family of nucleotide binding proteins, originally described in yeast as cell division cycle regulatory proteins. Septins are highly conserved in yeast, Drosophila, and mouse, and appear to regulate cytoskeletal organization. Disruption of septin function disturbs cytokinesis and results in large multinucleate or polyploid cells. This gene is highly expressed in brain and heart. Alternatively spliced transcript variants encoding different isoforms have been described for this gene. One of the isoforms (known as ARTS) is distinct; it is localized to the mitochondria, and has a role in apoptosis and cancer.
Description: This gene is a member of the septin gene family of nucleotide binding proteins, originally described in yeast as cell division cycle regulatory proteins. Septins are highly conserved in yeast, Drosophila, and mouse and appear to regulate cytoskeletal organization. Disruption of septin function disturbs cytokinesis and results in large multinucleate or polyploid cells. This gene is mapped to 22q11, the region frequently deleted in DiGeorge and velocardiofacial syndromes. A translocation involving the MLL gene and this gene has also been reported in patients with acute myeloid leukemia. Alternative splicing results in multiple transcript variants. The presence of a non-consensus polyA signal (AACAAT) in this gene also results in read-through transcription into the downstream neighboring gene (GP1BB; platelet glycoprotein Ib), whereby larger, non-coding transcripts are produced.
Description: This gene is a member of the septin family of nucleotide binding proteins, originally described in yeast as cell division cycle regulatory proteins. Septins are highly conserved in yeast, Drosophila, and mouse, and appear to regulate cytoskeletal organization. Disruption of septin function disturbs cytokinesis and results in large multinucleate or polyploid cells. Multiple alternatively spliced transcript variants encoding different isoforms have been found for this gene.
Description: This gene encodes a protein that is highly similar to the CDC10 protein of Saccharomyces cerevisiae. The protein also shares similarity with Diff 6 of Drosophila and with H5 of mouse. Each of these similar proteins, including the yeast CDC10, contains a GTP-binding motif. The yeast CDC10 protein is a structural component of the 10 nm filament which lies inside the cytoplasmic membrane and is essential for cytokinesis. This human protein functions in gliomagenesis and in the suppression of glioma cell growth, and it is required for the association of centromere-associated protein E with the kinetochore. Alternative splicing results in multiple transcript variants. Several related pseudogenes have been identified on chromosomes 5, 7, 9, 10, 11, 14, 17 and 19.
Description: This gene is a member of the septin gene family of nucleotide binding proteins, originally described in yeast as cell division cycle regulatory proteins. Septins are highly conserved in yeast, Drosophila, and mouse and appear to regulate cytoskeletal organization. Disruption of septin function disturbs cytokinesis and results in large multinucleate or polyploid cells. This gene is mapped to 22q11, the region frequently deleted in DiGeorge and velocardiofacial syndromes. A translocation involving the MLL gene and this gene has also been reported in patients with acute myeloid leukemia. Alternative splicing results in multiple transcript variants. The presence of a non-consensus polyA signal (AACAAT) in this gene also results in read-through transcription into the downstream neighboring gene (GP1BB; platelet glycoprotein Ib), whereby larger, non-coding transcripts are produced.
Description: This gene encodes a protein that is highly similar to the CDC10 protein of Saccharomyces cerevisiae. The protein also shares similarity with Diff 6 of Drosophila and with H5 of mouse. Each of these similar proteins, including the yeast CDC10, contains a GTP-binding motif. The yeast CDC10 protein is a structural component of the 10 nm filament which lies inside the cytoplasmic membrane and is essential for cytokinesis. This human protein functions in gliomagenesis and in the suppression of glioma cell growth, and it is required for the association of centromere-associated protein E with the kinetochore. Alternative splicing results in multiple transcript variants. Several related pseudogenes have been identified on chromosomes 5, 7, 9, 10, 11, 14, 17 and 19.
Description: This gene is a member of the septin family of nucleotide binding proteins, originally described in yeast as cell division cycle regulatory proteins. Septins are highly conserved in yeast, Drosophila, and mouse, and appear to regulate cytoskeletal organization. Disruption of septin function disturbs cytokinesis and results in large multinucleate or polyploid cells. Multiple alternatively spliced transcript variants encoding different isoforms have been found for this gene.
Description: This gene encodes a guanine-nucleotide binding protein and member of the septin family of cytoskeletal GTPases. Septins play important roles in cytokinesis, exocytosis, embryonic development, and membrane dynamics. Multiple transcript variants encoding different isoforms have been found for this gene.
Description: This gene is a member of the septin family of GTPases. Members of this family are required for cytokinesis and the maintenance of cellular morphology. This gene encodes a protein that can form homo- and heterooligomeric filaments, and may contribute to the formation of neurofibrillary tangles in Alzheimer's disease. Alternatively spliced transcript variants have been found but the full-length nature of these variants has not been determined. [provided by RefSeq, Dec 2012]
The Intra-assay Precision is determined when 3 samples with low, middle and high level of Human Anti-Anti-Sperm Antibody Antibody (Anti-AsAb) were tested on 3 different plates, 8 replicates in each plate
Description: This is Competitive Enzyme-linked immunosorbent assay for Antibody Detection.detection of Human Anti-Anti-Sperm Antibody Antibody (Anti-AsAb) in serum, plasma and other biological fluids.
Human Anti-Anti-Sperm Antibody Antibody (Anti-AsAb) ELISA Kit
The Intra-assay Precision is determined when 3 samples with low, middle and high level of Human Anti-Anti-Sperm Antibody Antibody (Anti-AsAb) were tested on 3 different plates, 8 replicates in each plate
Description: This is Competitive Enzyme-linked immunosorbent assay for Antibody Detection.detection of Human Anti-Anti-Sperm Antibody Antibody (Anti-AsAb) in serum, plasma and other biological fluids.
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GPR81 Antibody 
GPR81 antibody 
Rabbit Polyclonal antibody Anti-CRBN 
Polyclonal Goat Anti-GPR81 / FKSG80 Antibody 
GPR81 Conjugated Antibody 
GPR81 Blocking Peptide 
pDNR-Dual-GPR81 )
Polyclonal GPR81 Antibody (C-term) 
GPR81 ELISA KIT|Human )
Polyclonal FKSG80 / GPR81 Antibody (C-Terminus) )
Polyclonal FKSG80 / GPR81 Antibody (Extracellular Domain) )
Polyclonal FKSG80 / GPR81 Antibody (N-Terminus)  Antibody)
G Protein-Coupled Receptor 81 (GPR81) Antibody  (pORF))
Gpr81 ORF Vector (Rat) (pORF)  (pORF))
Gpr81 ORF Vector (Mouse) (pORF) )
Gpr81 sgRNA CRISPR Lentivector set (Rat) )
Gpr81 sgRNA CRISPR Lentivector set (Mouse) )
GPR81 sgRNA CRISPR Lentivector set (Human)  (Target 1))
Gpr81 sgRNA CRISPR Lentivector (Rat) (Target 1)  (Target 2))
Gpr81 sgRNA CRISPR Lentivector (Rat) (Target 2)  (Target 3))
Gpr81 sgRNA CRISPR Lentivector (Rat) (Target 3)  (Target 1))
Gpr81 sgRNA CRISPR Lentivector (Mouse) (Target 1)  (Target 2))
Gpr81 sgRNA CRISPR Lentivector (Mouse) (Target 2)  (Target 3))
Gpr81 sgRNA CRISPR Lentivector (Mouse) (Target 3)  (Target 1))
GPR81 sgRNA CRISPR Lentivector (Human) (Target 1)  (Target 2))
GPR81 sgRNA CRISPR Lentivector (Human) (Target 2)  (Target 3))
GPR81 sgRNA CRISPR Lentivector (Human) (Target 3)  (pPB-C-His))
GPR81 Protein Vector (Rat) (pPB-C-His)  (pPB-N-His))
GPR81 Protein Vector (Rat) (pPB-N-His)  (pPM-C-HA))
GPR81 Protein Vector (Rat) (pPM-C-HA)  (pPM-C-His))
GPR81 Protein Vector (Rat) (pPM-C-His)  (pPB-C-His))
GPR81 Protein Vector (Mouse) (pPB-C-His)  (pPB-N-His))
GPR81 Protein Vector (Mouse) (pPB-N-His)  (pPM-C-HA))
GPR81 Protein Vector (Mouse) (pPM-C-HA)  (pPM-C-His))
GPR81 Protein Vector (Mouse) (pPM-C-His) 
Gpr81 3'UTR Luciferase Stable Cell Line 
Gpr81 3'UTR GFP Stable Cell Line 
GPR81 3'UTR GFP Stable Cell Line 
GPR81 3'UTR Luciferase Stable Cell Line )
Gpr81 sgRNA CRISPR/Cas9 All-in-One Lentivector set (Rat) )
Gpr81 sgRNA CRISPR/Cas9 All-in-One Lentivector set (Mouse) )
GPR81 sgRNA CRISPR/Cas9 All-in-One Lentivector set (Human)  (Target 1))
Gpr81 sgRNA CRISPR/Cas9 All-in-One Lentivector (Rat) (Target 1)  (Target 2))
Gpr81 sgRNA CRISPR/Cas9 All-in-One Lentivector (Rat) (Target 2)  (Target 3))
Gpr81 sgRNA CRISPR/Cas9 All-in-One Lentivector (Rat) (Target 3)  (Target 1))
Gpr81 sgRNA CRISPR/Cas9 All-in-One Lentivector (Mouse) (Target 1)  (Target 2))
Gpr81 sgRNA CRISPR/Cas9 All-in-One Lentivector (Mouse) (Target 2)  (Target 3))
Gpr81 sgRNA CRISPR/Cas9 All-in-One Lentivector (Mouse) (Target 3)  (Target 1))
GPR81 sgRNA CRISPR/Cas9 All-in-One Lentivector (Human) (Target 1)  (Target 2))
GPR81 sgRNA CRISPR/Cas9 All-in-One Lentivector (Human) (Target 2)  (Target 3))
GPR81 sgRNA CRISPR/Cas9 All-in-One Lentivector (Human) (Target 3) 
Anti-Anti-SEPT6 antibody antibody 
Anti-Anti-SEPT9 Antibody antibody 
Anti-Anti-SEPT11 Antibody antibody 
Anti-Anti-SEPT4 Antibody antibody 
Anti-Anti-SEPT2 Antibody antibody 
Anti-Anti-SEPT5 Antibody antibody 
Anti-Anti-SEPT8 Antibody antibody 
Anti-Anti-SEPT7 Antibody antibody 
Anti-Anti-MARCH9 Antibody antibody 
Anti-Anti-MARCH8 Antibody antibody 
Anti-Anti-SEPT12 Antibody antibody 
Anti-Anti-MARCH6 Antibody antibody 
Anti-Anti-MARCH7 Antibody antibody 
Anti-Anti-SEPT3 Antibody antibody 
Anti-Anti-SEPT1 antibody antibody 
Anti-Anti-DDB1 Antibody  ELISA Kit)
Human Anti-Anti-Sperm Antibody Antibody (Anti-AsAb) ELISA Kit 
