Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human Valosin Containing Protein (VCP) in tissue homogenates, cell lysates and other biological fluids.

Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human Valosin Containing Protein (VCP) in tissue homogenates, cell lysates and other biological fluids.

Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human Valosin Containing Protein (VCP) in tissue homogenates, cell lysates and other biological fluids.

Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human Valosin Containing Protein (VCP) in tissue homogenates, cell lysates and other biological fluids.

Description: Enzyme-linked immunosorbent assay based on the Double-antibody Sandwich method for detection of Human Valosin Containing Protein (VCP) in samples from tissue homogenates, cell lysates and other biological fluids with no significant corss-reactivity with analogues from other species.

Description: Recombinant Human Valosin Containing Protein expressed in: E.coli

Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Rat Valosin Containing Protein (VCP) in Tissue homogenates and other biological fluids.

Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Rat Valosin Containing Protein (VCP) in Tissue homogenates and other biological fluids.

Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Rat Valosin Containing Protein (VCP) in Tissue homogenates and other biological fluids.

Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Rat Valosin Containing Protein (VCP) in Tissue homogenates and other biological fluids.

Description: Enzyme-linked immunosorbent assay based on the Double-antibody Sandwich method for detection of Rat Valosin Containing Protein (VCP) in samples from Tissue homogenates and other biological fluids. with no significant corss-reactivity with analogues from other species.

Description: A sandwich ELISA kit for detection of Valosin Containing Protein from Human in samples from blood, serum, plasma, cell culture fluid and other biological fluids.

Description: A sandwich ELISA kit for detection of Valosin Containing Protein from Rat in samples from blood, serum, plasma, cell culture fluid and other biological fluids.

Description: Supplied as a 0.2 μm filtered solution of 50mM TrisHCl，pH8.0.

Description: Supplied as a 0.2 μm filtered solution of 50mM TrisHCl，pH8.0.

Description: Supplied as a 0.2 μm filtered solution of 50mM TrisHCl，pH8.0.

Description: Supplied as a 0.2 μm filtered solution of 50mM TrisHCl，pH8.0.

Description: A Rabbit polyclonal antibody against Human, Rat, Pig Valosin Containing Protein (VCP)

Description: A Rabbit polyclonal antibody against Human, Rat, Pig Valosin Containing Protein (VCP). This antibody is labeled with HRP.

Description: A Rabbit polyclonal antibody against Human, Rat, Pig Valosin Containing Protein (VCP). This antibody is labeled with PE.

Description: A Rabbit polyclonal antibody against Human, Rat, Pig Valosin Containing Protein (VCP). This antibody is labeled with APC.

Description: A Rabbit polyclonal antibody against Human, Rat, Pig Valosin Containing Protein (VCP). This antibody is labeled with Biotin.

Description: A Rabbit polyclonal antibody against Human, Rat, Pig Valosin Containing Protein (VCP). This antibody is labeled with Cy3.

Description: A Rabbit polyclonal antibody against Human, Rat, Pig Valosin Containing Protein (VCP). This antibody is labeled with FITC.

Description: A Rabbit polyclonal antibody against Human, Rat, Pig Valosin Containing Protein (VCP). This antibody is labeled with APC-Cy7.

Description: Valosin-Containing Protein (VCP) is a nuclear protein that belongs to the AAA ATPase family. VCP is a putative ATP-binding protein involved in vesicle transport and fusion, 26S proteasome function, and assembly of peroxisomes. It is necessary for the fragmentation of Golgi stacks during mitosis and their reassembly after mitosis. VCP has been implicated in a number of cellular events that are regulated during mitosis, including homotypic membrane fusion, spindle pole body function, and ubiquitin-dependent protein degradation. VCP participates in the formation of the transitional endoplasmic reticulum (tER) and regulates E3 ubiquitin-protein ligase activity of RNF19A.

Description: VCPKMT Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 252 amino acids (1-229 a.a.) and having a molecular mass of 28.2kDa.

Description: Description of target: Necessary for the fragmentation of Golgi stacks during mitosis and for their reassembly after mitosis. Involved in the formation of the transitional endoplasmic reticulum (tER). The transfer of membranes from the endoplasmic reticulum to the Golgi apparatus occurs via 50-70 nm transition vesicles which derive from part-rough, part-smooth transitional elements of the endoplasmic reticulum (tER). Vesicle budding from the tER is an ATP-dependent process. The ternary complex containing UFD1L, VCP and NPLOC4 binds ubiquitinated proteins and is necessary for the export of misfolded proteins from the ER to the cytoplasm, where they are degraded by the proteasome. The NPLOC4-UFD1L-VCP complex regulates spindle disassembly at the end of mitosis and is necessary for the formation of a closed nuclear envelope. Regulates E3 ubiquitin-protein ligase activity of RNF19A. Component of the VCP/p97-AMFR/gp78 complex that participates in the final step of the sterol-mediated ubiquitination and endoplasmic reticulum-associated degradation (ERAD) of HMGCR. Also involved in DNA damage response: recruited to double-strand breaks (DSBs) sites in a RNF8- and RNF168-dependent manner and promotes the recruitment of TP53BP1 at DNA damage sites. Recruited to stalled replication forks by SPRTN: may act by mediating extraction of DNA polymerase eta (POLH) to prevent excessive translesion DNA synthesis and limit the incidence of mutations induced by DNA damage. Required for cytoplasmic retrotranslocation of stressed/damaged mitochondrial outer-membrane proteins and their subsequent proteasomal degradation. Essential for the maturation of ubiquitin-containing autophagosomes and the clearance of ubiquitinated protein by autophagy (PubMed:20104022).By similarity <p>Manually curated information which has been propagated from a related experimentally characterized protein.</p> <p><a href="/manual/evidences#ECO:0000250">More…</a></p> Manual assertion inferred from sequence similarity toiUniProtKB:P46462 (TERA_RAT)9 Publications <p>Manually curated information for which there is published experimental evidence.</p> <p><a href="/manual/evidences#ECO:0000269">More…</a></p> Manual assertion based on experiment iniRef.14"Physical and functional interaction between dorfin and valosin-containing protein that are colocalized in ubiquitylated inclusions in neurodegenerative disorders."_x005F_x005F_x000D_Ishigaki S., Hishikawa N., Niwa J., Iemura S., Natsume T., Hori S., Kakizuka A., Tanaka K., Sobue G._x005F_x005F_x000D_J. Biol. Chem. 279:51376-51385(2004) [PubMed] [Europe PMC] [Abstract]Cited for: FUNCTION, INTERACTION WITH RNF19A, IDENTIFICATION BY MASS SPECTROMETRY, SUBCELLULAR LOCATION, MUTAGENESIS OF LYS-524.Ref.18"Gp78, a membrane-anchored ubiquitin ligase, associates with Insig-1 and couples sterol-regulated ubiquitination to degradation of HMG CoA reductase."_x005F_x005F_x000D_Song B.L., Sever N., DeBose-Boyd R.A._x005F_x005F_x000D_Mol. Cell 19:829-840(2005) [PubMed] [Europe PMC] [Abstract]Cited for: INTERACTION WITH AMFR, FUNCTION, SUBCELLULAR LOCATION, MUTAGENESIS OF LYS-251 AND LYS-524.Ref.39"The AAA-ATPase p97 is essential for outer mitochondrial membrane protein turnover."_x005F_x005F_x000D_Xu S., Peng G., Wang Y., Fang S., Karbowski M._x005F_x005F_x000D_Mol. Biol. Cell 22:291-300(2011) [PubMed] [Europe PMC] [Abstract]Cited for: FUNCTION IN OMM PROTEIN TURNOVER.Ref.44"The ubiquitin-selective segregase VCP/p97 orchestrates the response to DNA double-strand breaks."_x005F_x005F_x000D_Meerang M., Ritz D., Paliwal S., Garajova Z., Bosshard M., Mailand N., Janscak P., Hubscher U., Meyer H., Ramadan K._x005F_x005F_x000D_Nat. Cell Biol. 13:1376-1382(2011) [PubMed] [Europe PMC] [Abstract]Cited for: FUNCTION.Ref.45"The AAA-ATPase VCP/p97 promotes 53BP1 recruitment by removing L3MBTL1 from DNA double-strand breaks."_x005F_x005F_x000D_Acs K., Luijsterburg M.S., Ackermann L., Salomons F.A., Hoppe T., Dantuma N.P._x005F_x005F_x000D_Nat. Struct. Mol. Biol. 18:1345-1350(2011) [PubMed] [Europe PMC] [Abstract]Cited for: FUNCTION, SUBCELLULAR LOCATION, INTERACTION WITH L3MBTL1.Ref.47"STT3B-dependent posttranslational N-glycosylation as a surveillance system for secretory protein."_x005F_x005F_x000D_Sato T., Sako Y., Sho M., Momohara M., Suico M.A., Shuto T., Nishitoh H., Okiyoneda T., Kokame K., Kaneko M., Taura M., Miyata M., Chosa K., Koga T., Morino-Koga S., Wada I., Kai H._x005F_x005F_x000D_Mol. Cell 47:99-110(2012) [PubMed] [Europe PMC] [Abstract]Cited for: FUNCTION IN ERAD PATHWAY.Ref.50"DVC1 (C1orf124) recruits the p97 protein segregase to sites of DNA damage."_x005F_x005F_x000D_Davis E.J., Lachaud C., Appleton P., Macartney T.J., Nathke I., Rouse J._x005F_x005F_x000D_Nat. Struct. Mol. Biol. 19:1093-1100(2012) [PubMed] [Europe PMC] [Abstract]Cited for: FUNCTION, INTERACTION WITH SPRTN.Ref.51"DVC1 (C1orf124) is a DNA damage-targeting p97 adaptor that promotes ubiquitin-dependent responses to replication blocks."_x005F_x005F_x000D_Mosbech A., Gibbs-Seymour I., Kagias K., Thorslund T., Beli P., Povlsen L., Nielsen S.V., Smedegaard S., Sedgwick G., Lukas C., Hartmann-Petersen R., Lukas J., Choudhary C., Pocock R., Bekker-Jensen S., Mailand N._x005F_x005F_x000D_Nat. Struct. Mol. Biol. 19:1084-1092(2012) [PubMed] [Europe PMC] [Abstract]Cited for: FUNCTION, SUBCELLULAR LOCATION, INTERACTION WITH SPRTN.Ref.62"VCP/p97 is essential for maturation of ubiquitin-containing autophagosomes and this function is impaired by mutations that cause IBMPFD."_x005F_x005F_x000D_Tresse E., Salomons F.A., Vesa J., Bott L.C., Kimonis V., Yao T.P., Dantuma N.P., Taylor J.P._x005F_x005F_x000D_Autophagy 6:217-227(2010) [PubMed] [Europe PMC] [Abstract]Cited for: CHARACTERIZATION OF VARIANTS IBMPFD1 HIS-155; SER-155 AND GLU-232, MUTAGENESIS OF GLU-305 AND GLU-578, FUNCTION. ;Species reactivity: Human;Application: ELISA;Assay info: Assay Methodology: Quantitative Sandwich Immunoassay;Sensitivity: < 0.112 ng/mL

Description: Description of target: This gene encodes a member of the AAA ATPase family of proteins. The encoded protein plays a role in protein degradation, intracellular membrane fusion, DNA repair and replication, regulation of the cell cycle, and activation of the NF-kappa B pathway. This protein forms a homohexameric complex that interacts with a variety of cofactors and extracts ubiquitinated proteins from lipid membranes or protein complexes. Mutations in this gene cause IBMPFD (inclusion body myopathy with paget disease of bone and frontotemporal dementia), ALS (amyotrophic lateral sclerosis) and Charcot-Marie-Tooth disease in human patients.;Species reactivity: Human;Application: ELISA;Assay info: Assay Methodology: Quantitative Sandwich ELISA;Sensitivity: 0.116 ng/mL

Description: Description of target: Necessary for the fragmentation of Golgi stacks during mitosis and for their reassembly after mitosis. Involved in the formation of the transitional endoplasmic reticulum (tER). The transfer of membranes from the endoplasmic reticulum to the Golgi apparatus occurs via 50-70 nm transition vesicles which derive from part-rough, part-smooth transitional elements of the endoplasmic reticulum (tER). Vesicle budding from the tER is an ATP-dependent process. The ternary complex containing UFD1L, VCP and NPLOC4 binds ubiquitinated proteins and is necessary for the export of misfolded proteins from the ER to the cytoplasm, where they are degraded by the proteasome. The NPLOC4-UFD1L-VCP complex regulates spindle disassembly at the end of mitosis and is necessary for the formation of a closed nuclear envelope. Regulates E3 ubiquitin-protein ligase activity of RNF19A. Also involved in DNA damage response: recruited to double-strand breaks (DSBs) sites in a RNF8- and RNF168-dependent manner and promotes the recruitment of TP53BP1 at DNA damage sites. Recruited to stalled replication forks by SPRTN: may act by mediating extraction of DNA polymerase eta (POLH) to prevent excessive translesion DNA synthesis and limit the incidence of mutations induced by DNA damage. Component of the VCP/p97-AMFR/gp78 complex that participates in the final step of the sterol-mediated ubiquitination and endoplasmic reticulum-associated degradation (ERAD) of HMGCR. Required for cytoplasmic retrotranslocation of stressed/damaged mitochondrial outer-membrane proteins and their subsequent proteasomal degradation. Essential for the maturation of ubiquitin-containing autophagosomes and the clearance of ubiquitinated protein by autophagy.;Species reactivity: Rat;Application: ;Assay info: Assay Methodology: Quantitative Sandwich Immunoassay;Sensitivity: < 0.115 ng/mL

Description: A polyclonal antibody against VCP. Recognizes VCP from Human, Mouse, Rat. This antibody is Unconjugated. Tested in the following application: WB, IHC, ELISA;WB:1/500-1/2000.IHC:1/100-1/300.ELISA:1/40000

Description: A polyclonal antibody against VCP. Recognizes VCP from Human, Mouse, Rat. This antibody is Unconjugated. Tested in the following application: ELISA, WB, IHC

Description: A polyclonal antibody against VCP. Recognizes VCP from Human. This antibody is Unconjugated. Tested in the following application: ELISA, WB, IHC; Recommended dilution: WB:1:500-1:5000, IHC:1:20-1:200

Description: A polyclonal antibody against vcp. Recognizes vcp from Zebrafish. This antibody is Unconjugated. Tested in the following application: ELISA, WB; Recommended dilution: WB:1:500-1:5000

Description: VCP Antibody detects endogenous levels of VCP.

Description: VCP Antibody detects endogenous levels of total VCP.

Description: Affinity purified rabbit polyclonal VCP antibody

Description: Rabbit polyclonal VCP antibody

Description: Affinity purified Rabbit polyclonal VCP antibody